Selection of novel affinity-matured human chondroitin sulfate proteoglycan 4 antibody fragments by yeast display.

ABSTRACT: Chondroitin sulfate proteoglycan 4 (CSPG4) is a promising target for cancer immunotherapy due to its high level of expression in a number of malignant tumors, and its essential role in tumor growth and progression. Clinical application of CSPG4-targeting immunotherapies is hampered by the lack of fully human high-affinity CSPG4 antibodies or antibody fragments. To overcome this limitation, we performed affinity maturation on a novel human CSPG4 single-chain Fv fragment (scFv) using the random mutagenesis approach and screened for improved variants from a yeast display library using a modified whole-cell panning method followed by fluorescence-activated cell sorting. After six rounds of panning and sorting, the top seven mutant scFvs were isolated and their binding affinities were character

SUBMITTER: Yu X 

PROVIDER: S-EPMC5914443 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature