Probing the application range and selectivity of a differential mobility spectrometry-mass spectrometry platform for metabolomics.

ABSTRACT: Metabolomics applications of differential mobility spectrometry (DMS)-mass spectrometry (MS) have largely concentrated on targeted assays and the removal of isobaric or chemical interferences from the signals of a small number of analytes. In the work reported here, we systematically investigated the application range of a DMS-MS method for metabolomics using more than 800 authentic metabolite standards as the test set. The coverage achieved with the DMS-MS platform was comparable to that achieved with chromatographic methods. High orthogonality was observed between hydrophilic interaction liquid chromatography and the 2-propanol-mediated DMS separation, and previously observed similarities were confirmed for the DMS platform and reversed-phase liquid chromatography. We describe the chemic

SUBMITTER: Wernisch S 

PROVIDER: S-EPMC5915368 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature