Excision of the doubly methylated base N⁴,5-dimethylcytosine from DNA by Escherichia coli Nei and Fpg proteins.

ABSTRACT: Cytosine (C) in DNA is often modified to 5-methylcytosine (m⁵C) to execute important cellular functions. Despite the significance of m⁵C for epigenetic regulation in mammals, damage to m⁵C has received little attention. For instance, almost no studies exist on erroneous methylation of m⁵C by alkylating agents to doubly or triply methylated bases. Owing to chemical evidence, and because many prokaryotes express methyltransferases able to convert m⁵C into N⁴,5-dimethylcytosine (m ^N4,5C) in DNA, m ^N4,5C is probably present in vivo We screened a series of glycosylases from prokaryotic to human and found significant DNA incision activity of the Escherichia

SUBMITTER: Alexeeva M 

PROVIDER: S-EPMC5915725 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature