Chiral probes for α₁-AGP reporting by species-specific induced circularly polarised luminescence.

ABSTRACT: Luminescence spectroscopy has been used to monitor the selective and reversible binding of pH sensitive, macrocyclic lanthanide complexes, [LnL¹], to the serum protein α₁-AGP, whose concentration can vary significantly in response to inflammatory processes. On binding α₁-AGP, a very strong induced circularly-polarised europium luminescence signal was observed that was of opposite sign for human and bovine variants of α₁-AGP - reflecting the differences in the chiral environment of their drug-binding pockets. A mixture of [EuL¹] and [TbL¹] complexes allowed the ratiometric monitoring of α₁-AGP levels in serum. Moreover, competitive displacement of [EuL¹] from the protein by ce

SUBMITTER: Shuvaev S 

PROVIDER: S-EPMC5915836 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature