Dataset Information

Red pulp macrophages in the human spleen are a distinct cell population with a unique expression of Fc-? receptors.

ABSTRACT: Tissue-resident macrophages in the spleen play a major role in the clearance of immunoglobulin G (IgG)-opsonized blood cells, as occurs in immune thrombocytopenia (ITP) and autoimmune hemolytic anemia (AIHA). Blood cells are phagocytosed via the Fc-? receptors (Fc?Rs), but little is known about the Fc?R expression on splenic red pulp macrophages in humans, with only a few previous studies that showed conflicting results. We developed a novel method to specifically isolate red pulp macrophages from 82 human spleens. Surface expression of various receptors and phagocytic capacity was analyzed by flow cytometry and immunofluorescence of tissue sections. Red pulp macrophages were distinct from splenic monocytes and blood monocyte-derived macrophages on various surface markers. Human red pulp macrophages predominantly expressed the low-affinity receptors Fc?RIIa and Fc?RIIIa. In contrast to blood monocyte-derived macrophages, red pulp macrophages did not express the inhibitory Fc?RIIb. Red pulp macrophages expressed very low levels of the high-affinity receptor Fc?RI. Messenger RNA transcript analysis confirmed this expression pattern. Unexpectedly and despite these differences in Fc?R expression, phagocytosis of IgG-opsonized blood cells by red pulp macrophages was dependent on the same Fc?Rs as phagocytosis by blood monocyte-derived macrophages, especially in regarding the response to IV immunoglobulin. Concluding, we show the distinct nature of splenic red pulp macrophages in human subjects. Knowledge on the Fc?R expression and usage of these cells is important for understanding and improving treatment strategies for autoimmune diseases such as ITP and AIHA.

SUBMITTER: Nagelkerke SQ

PROVIDER: S-EPMC5916003 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Red pulp macrophages in the human spleen are a distinct cell population with a unique expression of Fc-γ receptors.

Nagelkerke Sietse Q SQ Bruggeman Christine W CW den Haan Joke M M JMM Mul Erik P J EPJ van den Berg Timo K TK van Bruggen Robin R Kuijpers Taco W TW

Blood advances 20180401 8

Tissue-resident macrophages in the spleen play a major role in the clearance of immunoglobulin G (IgG)-opsonized blood cells, as occurs in immune thrombocytopenia (ITP) and autoimmune hemolytic anemia (AIHA). Blood cells are phagocytosed via the Fc-γ receptors (FcγRs), but little is known about the FcγR expression on splenic red pulp macrophages in humans, with only a few previous studies that showed conflicting results. We developed a novel method to specifically isolate red pulp macrophages fr ...[more]

PMID: 29692344

Similar Datasets

Project description:Thiele2013 - Spleen cells in red pulp The model of spleen cells in red pulp metabolism is derived from the community-driven global reconstruction of human metabolism (version 2.02, MODEL1109130000 ). This model is described in the article: A community-driven global reconstruction of human metabolism. Thiele I, et al . Nature Biotechnology Abstract: Multiple models of human metabolism have been reconstructed, but each represents only a subset of our knowledge. Here we describe Recon 2, a community-driven, consensus 'metabolic reconstruction', which is the most comprehensive representation of human metabolism that is applicable to computational modeling. Compared with its predecessors, the reconstruction has improved topological and functional features, including ~2x more reactions and ~1.7x more unique metabolites. Using Recon 2 we predicted changes in metabolite biomarkers for 49 inborn errors of metabolism with 77% accuracy when compared to experimental data. Mapping metabolomic data and drug information onto Recon 2 demonstrates its potential for integrating and analyzing diverse data types. Using protein expression data, we automatically generated a compendium of 65 cell type-specific models, providing a basis for manual curation or investigation of cell-specific metabolic properties. Recon 2 will facilitate many future biomedical studies and is freely available at http://humanmetabolism.org/. This model is hosted on BioModels Database and identified by: MODEL1310110060 . To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models . To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Dataset Information

Red pulp macrophages in the human spleen are a distinct cell population with a unique expression of Fc-? receptors.

Publications

Red pulp macrophages in the human spleen are a distinct cell population with a unique expression of Fc-γ receptors.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure