DREADDed microglia in pain: Implications for spinal inflammatory signaling in male rats.

ABSTRACT: The absence of selective pharmacological tools is a major barrier to the in vivo study of microglia. To address this issue, we developed a G_q- and G_i-coupled Designer Receptor Exclusively Activated by a Designer Drug (DREADD) to enable selective stimulation or inhibition of microglia, respectively. DREADDs under a CD68 (microglia/macrophage) promoter were intrathecally transfected via an AAV9 vector. Naïve male rats intrathecally transfected with G_q (stimulatory) DREADDs exhibited significant allodynia following intrathecal administration of the DREADD-selective ligand clozapine-N-oxide (CNO), which was abolished by intrathecal interleukin-1 receptor antagonist. Chronic constriction injury-induced allodynia was attenuated by intrathecal CNO in male rats int

SUBMITTER: Grace PM 

PROVIDER: S-EPMC5916033 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature