Isolation and characterization of a monoclonal antibody containing an extra heavy-light chain Fab arm.

ABSTRACT: Isolation and characterization of monoclonal antibody (mAb) variants to understand the impact of their structure on function is a typical activity during early-stage candidate selection that contributes to derisking clinical development. In particular, efforts are devoted to characterizing oligomeric variants, owing to their potential immunogenic nature. We report here a mAb variant consisting of a canonical mAb monomer associated in a non-covalent fashion with an antigen-binding fragment (Fab) arm amputated from its Fc domain. The truncated heavy chain is encoded in the cell line genome and is the likely product of a genomic recombination during cell line generation. The addition of the Fab arm results in severe loss of potency, indicating its interaction with the Fab domain of the monome

SUBMITTER: Boyd D 

PROVIDER: S-EPMC5916558 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature