A novel field-based molecular assay to detect validated artemisinin-resistant k13 mutants.

ABSTRACT:

Background

Given the risk of artemisinin resistance spreading from the Greater Mekong sub-region, prospective monitoring in sub-Saharan Africa should be expedited. Molecular biology techniques used for monitoring rely on the detection of k13 validated mutants by using PCR and Sanger sequencing approach, usually not available in malaria endemic areas.

Methods

A semi-automated workflow based on the easyMAG^® platform and the Argene Solution^® (bioMérieux, Marcy l'Etoile, France) as a field-based surveillance tool operable at national level was developed in four steps. Clinical and analytical performances of this tool detecting five of the most frequent and validated k13 mutants (Y493H, I543T, R539T, F446I and C580Y) from dried blood spots (DBS) were compare

SUBMITTER: Vachot-Ganee L 

PROVIDER: S-EPMC5916714 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature