Precursor ion exclusion for enhanced identification of plasma biomarkers.

ABSTRACT:

Purpose

Our study aims to establish a plasma biomarker analysis workflow, with fewer fractionation steps, for enhanced identification of plasma biomarkers by precursor ion exclusion (PIE).

Experimental design

Plasma samples were depleted for highly abundant proteins, then further fractionated by molecular weight (MW), before trypsinization for LTQ-Orbitrap mass analysis. Data-dependent acquisition (DDA) was used for baseline analysis. PIE involves the re-injection of samples with exclusion of the previously identified peptides. We compared analyses using multiple PIE iterations, compared to DDA, for plasma interrogation

Results

A higher percentage of unique plasma peptides was identified with PIE, compared to DDA. The first PIE iteration reveals an increase of 75-112%

SUBMITTER: Wu WW 

PROVIDER: S-EPMC5916817 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature