Autophagy inhibition improves the cytotoxic effects of receptor tyrosine kinase inhibitors.

ABSTRACT:

Background

A growing field of evidence suggests the involvement of oncogenic receptor tyrosine kinases (RTKs) in cell transformation. Deregulated activity of RTKs in tumors can determine disease progression and therapeutic responses in several types of cancer, including neuroblastoma (NB). Therefore, RTKs targeting is a worthwhile challenge for the oncologists. Nevertheless, acquired resistance to RTK inhibitors (RTKi) remains a serious problem. Autophagy activation is among the possible obstacles for good efficacy of the therapy with RTKi.

Methods

Under different treatment conditions we measured autophagic flux using immunoblot and immunofluorescence assays. Death induction was validated by trypan blue exclusion assay and FACS analysis (calcein-AM/propidium iodide). The NB

SUBMITTER: Aveic S 

PROVIDER: S-EPMC5916832 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature