Targeted mutagenesis in wheat microspores using CRISPR/Cas9.

ABSTRACT: CRISPR/Cas9 genome editing is a transformative technology that will facilitate the development of crops to meet future demands. However, application of gene editing is hindered by the long life cycle of many crop species and because desired genotypes generally require multiple generations to achieve. Single-celled microspores are haploid cells that can develop into double haploid plants and have been widely used as a breeding tool to generate homozygous plants within a generation. In this study, we combined the CRISPR/Cas9 system with microspore technology and developed an optimized haploid mutagenesis system to induce genetic modifications in the wheat genome. We investigated a number of factors that may affect the delivery of CRISPR/Cas9 reagents into microspores and found that electropo

SUBMITTER: Bhowmik P 

PROVIDER: S-EPMC5916876 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature