Molecular design of radiocopper-labelled Affibody molecules.

ABSTRACT: The use of long-lived positron emitters ⁶⁴Cu or ⁶¹Cu for labelling of Affibody molecules may improve breast cancer patients' stratification for HER-targeted therapy. Previous animal studies have shown that the use of triaza chelators for ⁶⁴Cu labelling of synthetic Affibody molecules is suboptimal. In this study, we tested a hypothesis that the use of cross-bridged chelator, CB-TE2A, in combination with Gly-Glu-Glu-Glu spacer for labelling of Affibody molecules with radiocopper would improve imaging contrast. CB-TE2A was coupled to the N-terminus of synthetic Affibody molecules extended either with a glycine (designation CB-TE2A-G-ZHER2:342) or Gly-Glu-Glu-Glu spacer (CB-TE2A-GEEE-ZHER2:342). Biodistribution and targeting properties of ⁶⁴Cu-CB-TE

SUBMITTER: Tolmachev V 

PROVIDER: S-EPMC5916907 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature