Salinity effect on the metabolic pathway and microbial function in phenanthrene degradation by a halophilic consortium.

ABSTRACT: With the close relationship between saline environments and industry, polycyclic aromatic hydrocarbons (PAHs) accumulate in saline/hypersaline environments. Therefore, PAHs degradation by halotolerant/halophilic bacteria has received increasing attention. In this study, the metabolic pathway of phenanthrene degradation by halophilic consortium CY-1 was first studied which showed a single upstream pathway initiated by dioxygenation at the C1 and C2 positions, and at several downstream pathways, including the catechol pathway, gentisic acid pathway and protocatechuic acid pathway. The effects of salinity on the community structure and expression of catabolic genes were further studied by a combination of high-throughput sequencing, catabolic gene clone library and real-time PCR. Pure culture

SUBMITTER: Wang C 

PROVIDER: S-EPMC5918149 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature