The Coding Regions of Germline mRNAs Confer Sensitivity to Argonaute Regulation in C. elegans.

ABSTRACT: Protein-coding genes undergo a wide array of regulatory interactions with factors that engage non-coding regions. Open reading frames (ORFs), in contrast, are thought to be constrained by coding function, precluding a major role in gene regulation. Here, we explore Piwi-interacting (pi)RNA-mediated transgene silencing in C. elegans and show that marked differences in the sensitivity to piRNA silencing map to the endogenous sequences within transgene ORFs. Artificially increasing piRNA targeting within the ORF of a resistant transgene can lead to a partial yet stable reduction in expression, revealing that piRNAs not only silence but can also "tune" gene expression. Our findings support a model that involves a temporal element to mRNA regulation by germline Argonautes, likely prior to trans

SUBMITTER: Seth M 

PROVIDER: S-EPMC5918280 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature