High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells.

ABSTRACT: Investigation of human muscle regeneration requires robust methods to purify and transplant muscle stem and progenitor cells that collectively constitute the human satellite cell (HuSC) pool. Existing approaches have yet to make HuSCs widely accessible for researchers, and as a result human muscle stem cell research has advanced slowly. Here, we describe a robust and predictable HuSC purification process that is effective for each human skeletal muscle tested and the development of storage protocols and transplantation models in dystrophin-deficient and wild-type recipients. Enzymatic digestion, magnetic column depletion, and 6-marker flow-cytometric purification enable separation of 10⁴ highly enriched HuSCs per gram of muscle. Cryostorage of HuSCs preserves viability, phenotyp

SUBMITTER: Garcia SM 

PROVIDER: S-EPMC5918346 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature