Accurate and easy method for systemin quantification and examining metabolic changes under different endogenous levels.

ABSTRACT:

Background

Systemin has been extensively studied since it was discovered and is described as a peptidic hormone in tomato plants and other Solanaceae. Jasmonic acid and systemin are proposed to act through a positive feed-back loop with jasmonic acid, playing synergistic roles in response to both wounding and insect attack. Despite its biological relevance, most studies regarding the function of systemin in defence have been studied via PROSYSTEMIN (PROSYS) gene expression, which encodes the propeptide prosystemin that is later cleaved to systemin (SYS). Interestingly, hardly any studies have been based on quantification of the peptide.

Results

In this study, a simple and accurate method for systemin quantification was developed to understand its impact on plan

SUBMITTER: Pastor V 

PROVIDER: S-EPMC5918566 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature