Prospective Isolation of ISL1⁺ Cardiac Progenitors from Human ESCs for Myocardial Infarction Therapy.

ABSTRACT: The LIM-homeodomain transcription factor ISL1 marks multipotent cardiac progenitors that give rise to cardiac muscle, endothelium, and smooth muscle cells. ISL1⁺ progenitors can be derived from human pluripotent stem cells, but the inability to efficiently isolate pure populations has limited their characterization. Using a genetic selection strategy, we were able to highly enrich ISL1⁺ cells derived from human embryonic stem cells. Comparative quantitative proteomic analysis of enriched ISL1⁺ cells identified ALCAM (CD166) as a surface marker that enabled the isolation of ISL1⁺ progenitor cells. ALCAM⁺/ISL1⁺ progenitors are multipotent and differentiate into cardiomyocytes, endothelial cells, and smooth muscle cells. Transp

SUBMITTER: Ghazizadeh Z 

PROVIDER: S-EPMC5918615 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature