The presence of human mesenchymal stem cells of renal origin in amniotic fluid increases with gestational time.

ABSTRACT: BACKGROUND:Established therapies for managing kidney dysfunction such as kidney dialysis and transplantation are limited due to the shortage of compatible donated organs and high costs. Stem cell-based therapies are currently under investigation as an alternative treatment option. As amniotic fluid is composed of fetal urine harboring mesenchymal stem cells (AF-MSCs), we hypothesized that third-trimester amniotic fluid could be a novel source of renal progenitor and differentiated cells. METHODS:Human third-trimester amniotic fluid cells (AFCs) were isolated and cultured in distinct media. These cells were characterized as renal progenitor cells with respect to cell morphology, cell surface marker expression, transcriptome and differentiation into chondrocytes, osteoblasts and adipocytes.

SUBMITTER: Rahman MS 

PROVIDER: S-EPMC5918774 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature