Development of a statistical model for cervical cancer cell death with irreversible electroporation in vitro.

ABSTRACT:

Purpose

The aim of this study was to develop a statistical model for cell death by irreversible electroporation (IRE) and to show that the statistic model is more accurate than the electric field threshold model in the literature using cervical cancer cells in vitro.

Methods

HeLa cell line was cultured and treated with different IRE protocols in order to obtain data for modeling the statistical relationship between the cell death and pulse-setting parameters. In total, 340 in vitro experiments were performed with a commercial IRE pulse system, including a pulse generator and an electric cuvette. Trypan blue staining technique was used to evaluate cell death after 4 hours of incubation following IRE treatment. Peleg-Fermi model was used in the study to build the statistical r

SUBMITTER: Yang Y 

PROVIDER: S-EPMC5919048 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature