DNA polymerase ?: A missing link of the base excision repair machinery in mammalian mitochondria.

ABSTRACT: Mitochondrial genome integrity is fundamental to mammalian cell viability. Since mitochondrial DNA is constantly under attack from oxygen radicals released during ATP production, DNA repair is vital in removing oxidatively generated lesions in mitochondrial DNA, but the presence of a strong base excision repair system has not been demonstrated. Here, we addressed the presence of such a system in mammalian mitochondria involving the primary base lesion repair enzyme DNA polymerase (pol) ?. Pol ? was localized to mammalian mitochondria by electron microscopic-immunogold staining, immunofluorescence co-localization and biochemical experiments. Extracts from purified mitochondria exhibited base excision repair activity that was dependent on pol ?. Mitochondria from pol ?-deficient mouse fibroblasts had compromised DNA repair and showed elevated levels of superoxide radicals after hydrogen peroxide treatment. Mitochondria in pol ?-deficient fibroblasts displayed altered morphology by electron microscopy. These results indicate that mammalian mitochondria contain an efficient base lesion repair system mediated in part by pol ? and thus pol ? plays a role in preserving mitochondrial genome stability.

SUBMITTER: Prasad R 

PROVIDER: S-EPMC5919216 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature