Electrostatic Control of Polymer Translocation Speed through α‑Hemolysin Protein Pore.

ABSTRACT: The electrostatic origins behind the speed of translocation of a uniformly charged flexible macromolecule through α-hemolysin (αHL) protein pores under a voltage are investigated using variations in pH and electrolyte concentration. We have measured durations of successful threading of poly(styrenesulfonate) through αHL at two different pH conditions, pH 4.5 and pH 7.5, under various salt concentration conditions. Salt concentrations in the donor (cis) and the recipient (trans) compartments influence the polymer translocation dynamics differently, depending on pH. At both pH 4.5 and pH 7.5, decreasing the cis salt concentration, c_s,cis , results in faster polymer translocations. On the other hand, a decrease in trans salt c

SUBMITTER: Jeon BJ 

PROVIDER: S-EPMC5919254 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature