Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags.

ABSTRACT: Milk oligosaccharides (OS) are a key factor that influences the infant gut microbial composition, and their importance in promoting healthy infant development and disease prevention is becoming increasingly apparent. Investigating the structures, properties, and sources of these compounds requires a host of complementary analytical techniques. Relative compound quantification by mass spectral analysis of isobarically labeled samples is a relatively new technique that has been used mainly in the proteomics field. Glycomics applications have so far focused on analysis of protein-linked glycans, while analysis of free milk OS has previously been conducted only on analytical standards. In this paper, we extend the use of isobaric glycan tags to the analysis of bovine milk OS by presenting a me

SUBMITTER: Robinson RC 

PROVIDER: S-EPMC5919578 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature