Growth of Caenorhabditis elegans in Defined Media Is Dependent on Presence of Particulate Matter.

ABSTRACT: Caenorhabditis elegans are typically cultured in a monoxenic medium consisting of live bacteria. However, this introduces a secondary organism to experiments, and restricts the manipulation of the nutritional environment. Due to the intricate link between genes and environment, greater control and understanding of nutritional factors are required to push the C. elegans field into new areas. For decades, attempts to develop a chemically defined, axenic medium as an alternative for culturing C. elegans have been made. However, the mechanism by which the filter feeder C. elegans obtains nutrients from these liquid media is not known. Using a fluorescence-activated cell sorting based approach, we demonstrate growth in all past axenic C. elegans media to be de

SUBMITTER: Flavel MR 

PROVIDER: S-EPMC5919726 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature