Transient elevation of cytoplasmic calcium ion concentration at a single cell level precedes morphological changes of epidermal keratinocytes during cornification.

ABSTRACT: Epidermal keratinocytes achieve sequential differentiation from basal to granular layers, and undergo a specific programmed cell death, cornification, to form an indispensable barrier of the body. Although elevation of the cytoplasmic calcium ion concentration ([Ca²⁺]_i) is one of the factors predicted to regulate cornification, the dynamics of [Ca²⁺]_i in epidermal keratinocytes is largely unknown. Here using intravital imaging, we captured the dynamics of [Ca²⁺]_i in mouse skin. [Ca²⁺]_i was elevated in basal cells on the second time scale in three spatiotemporally distinct patterns. The transient elevation of [Ca²⁺]_i also occurred at the most apical granular layer at a single cell l

SUBMITTER: Murata T 

PROVIDER: S-EPMC5919969 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature