Novel analysis of the Harderian gland transcriptome response to Newcastle disease virus in two inbred chicken lines.

ABSTRACT: Behind each eye of the chicken resides a unique lymph tissue, the Harderian gland, for which RNA sequencing (RNA-seq) analysis is novel. We characterized the response of this tissue to Newcastle disease virus (NDV) in two inbred lines with different susceptibility to NDV across three time points. Three-week-old relatively resistant (Fayoumi) and relatively susceptible (Leghorn) birds were inoculated with a high-titered (10⁷EID₅₀) La Sota strain of NDV via an oculonasal route. At 2, 6, and 10 days post infection (dpi) Harderian glands were collected and analyzed via RNA-seq. The Fayoumi had significantly more detectable viral transcripts in the Harderian gland at 2 dpi than the Leghorn, but cleared the virus by 6 dpi. At all three time points, few genes were declared d

SUBMITTER: Deist MS 

PROVIDER: S-EPMC5920083 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature