Cardiopulmonary bypass reduces myocardial oxidative stress, inflammation and increases c-kit⁺CD45^- cell population in newborns.

ABSTRACT:

Background

The aim of this study was to characterize the influence of cardiopulmonary bypass (CPB) on myocardial remodeling in newborns and children.

Methods

Biopsies from the right atrium were taken before and after CPB from 4 newborns (5-11 days old) and 7 children (8 months-16 years old). Immunostainings on 10 µm heart tissue frozen sections were performed to detect c-kit⁺ cells, leukocytes (CD45⁺ cells), Ki67⁺ cycling cells. The percentage of 8-hydroxy-guanosine (8-dOHG)⁺cardiomyocytes and non-cardiomyocytes [(8-dOHG)⁺-index] were determined to quantify oxidative stress.

Results

Δ c-kit⁺CD45^- cells (resident cardiac stem cells) were increased in newborns (2.2 ± 1.9/mm²) and dec

SUBMITTER: Petersen J 

PROVIDER: S-EPMC5921779 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature