Porous Substrates Promote Endothelial Migration at the Expense of Fibronectin Fibrillogenesis.

ABSTRACT: Porous substrates have gained increased usage in cell studies and tissue mimetic applications because they can partition distinct cell types while still allowing important biochemical crosstalk. In the presented work, we investigated how porous substrates with micron and submicron features influence early cell migration and the associated ECM establishment, which can critically affect the rate of cell coverage on the substrate and the ensuing tissue organization. We showed through time-lapse microscopy that cell speed and migratory distance on membranes with 0.5 μm pores were nearly two-fold of those observed on nonporous membranes, while values on membranes with 3.0 μm pores fell in between. Although the cell directionality ratio and the persistence time was unaffected by the presence of

SUBMITTER: Chung HH 

PROVIDER: S-EPMC5921834 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature