Metaproteomics of Colonic Microbiota Unveils Discrete Protein Functions among Colitic Mice and Control Groups.

ABSTRACT: Metaproteomics can greatly assist established high-throughput sequencing methodologies to provide systems biological insights into the alterations of microbial protein functionalities correlated with disease-associated dysbiosis of the intestinal microbiota. Here, the authors utilize the well-characterized murine T cell transfer model of colitis to find specific changes within the intestinal luminal proteome associated with inflammation. MS proteomic analysis of colonic samples permitted the identification of ≈10 000-12 000 unique peptides that corresponded to 5610 protein clusters identified across three groups, including the colitic Rag1^-/- T cell recipients, isogenic Rag1^-/- controls, and wild-type mice. The authors demonstrate that the colitic mice exhibited a sig

SUBMITTER: Moon C 

PROVIDER: S-EPMC5921860 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature