Androgen receptor and heat shock protein 27 co-regulate the malignant potential of molecular apocrine breast cancer.

ABSTRACT:

Background

The most striking feature of molecular apocrine breast cancer (MABC) is the expression of androgen receptor (AR). We report here the mechanism of the AR in regulating the behavior of MABC.

Methods

The MABC cell line, MDA-MB-453, and the nonMABC cell line, MCF7, were used in this study. The effect of dihydrotestosterone (DHT) and heat shock protein 27 (HSP27) on cell proliferation was quantified using the cell counter kit-8 (CCK8) and clonogenic assays in vitro and by a xenograft tumor model in vivo. The expression of the AR and HSP27 was analyzed using western blot, qPCR, and immunofluorescence assays. Complexes of the AR and HSP27 were detected by co-immunoprecipitation (Co-IP).

Results

In MDA-MB-453 cells, DHT promoted cell proliferation and stimulated AR

SUBMITTER: Liu X 

PROVIDER: S-EPMC5921986 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature