Regulation of calretinin in malignant mesothelioma is mediated by septin 7 binding to the CALB2 promoter.

ABSTRACT:

Background

The calcium-binding protein calretinin (gene name: CALB2) is currently considered as the most sensitive and specific marker for the diagnosis of malignant mesothelioma (MM). MM is a very aggressive tumor strongly linked to asbestos exposure and with no existing cure so far. The mechanisms of calretinin regulation, as well as its distinct function in MM are still poorly understood.

Methods

We searched for transcription factors binding to the CALB2 promoter and modulating calretinin expression. For this, DNA-binding assays followed by peptide shotgun-mass spectroscopy analyses were used. CALB2 promoter activity was assessed by dual-luciferase reporter assays. Furthermore, we analyzed the effects of CALB2 promoter-binding proteins by lentiviral-mediated overexpressio

SUBMITTER: Blum W 

PROVIDER: S-EPMC5922012 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature