A robust method for RNA extraction and purification from a single adult mouse tendon.

ABSTRACT:

Background

Mechanistic understanding of tendon molecular and cellular biology is crucial toward furthering our abilities to design new therapies for tendon and ligament injuries and disease. Recent transcriptomic and epigenomic studies in the field have harnessed the power of mouse genetics to reveal new insights into tendon biology. However, many mouse studies pool tendon tissues or use amplification methods to perform RNA analysis, which can significantly increase the experimental costs and limit the ability to detect changes in expression of low copy transcripts.

Methods

Single Achilles tendons were harvested from uninjured, contralateral injured, and wild type mice between three and five months of age, and RNA was extracted. RNA Integrity Number (RIN) and concentration w

SUBMITTER: Grinstein M 

PROVIDER: S-EPMC5922231 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature