Gut microbial RNA and DNA analysis predicts hospitalizations in cirrhosis.

ABSTRACT:

Background

Cirrhosis is associated with gut microbial changes, but current 16S rDNA techniques sequence both dead and live bacteria. We aimed to determine the rRNA content compared with DNA from the same stool sample to evaluate cirrhosis progression and predict hospitalizations.

Methods

Cirrhotics and controls provided stool for RNA and DNA analysis. Comparisons were made between cirrhotics/controls and within cirrhosis (compensated/decompensated, infected/uninfected, renal dysfunction/not, rifaximin use/not) with respect to DNA and RNA bacterial content using linear discriminant analysis. A separate group was treated with omeprazole for 14 days with longitudinal microbiota evaluation. Patients were followed for 90 days for hospitalizations. Multivariable models for hospita

SUBMITTER: Bajaj JS 

PROVIDER: S-EPMC5922291 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature