Epigenetic hypomethylation and upregulation of NLRC4 and NLRP12 in Kawasaki disease.

ABSTRACT:

Introduction

Kawasaki disease (KD) is a type of childhood febrile systemic vasculitis. Inflammasomes control inflammatory signaling and are related with the development of KD. In this study, we performed a survey of transcripts and global DNA methylation levels of inflammasome sensors of NOD-like receptors (NLRs) and the downstream interleukin 1β (IL-1β).

Materials and methods

In this study, for the chip studies, we recruited a total of 18 KD patients, who we analyzed before receiving intravenous immunoglobulin (IVIG) and at least 3 weeks after IVIG treatment, as well as 36 non-fever controls by Illumina HumanMethylation 450 BeadChip and Affymetrix GeneChip® Human Transcriptome Array 2.0. A separate group of 78 subjects was performed for real-time quantitative PCR validation

SUBMITTER: Huang YH 

PROVIDER: S-EPMC5922368 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature