Myoferlin regulates epithelial cancer cell plasticity and migration through autocrine TGF-β1 signaling.

ABSTRACT: Epithelial cancer cells can undergo an epithelial-mesenchymal transition (EMT), a complex genetic program that enables cells to break free from the primary tumor, breach the basement membrane, invade through the stroma and metastasize to distant organs. Myoferlin (MYOF), a protein involved in plasma membrane function and repair, is overexpressed in several invasive cancer cell lines. Depletion of myoferlin in the human breast cancer cell line MDA-MB-231 (MDA-231^MYOFKD) reduced migration and invasion and caused the cells to revert to an epithelial phenotype. To test if this mesenchymal-epithelial transition was durable, MDA-231^MYOFKD cells were treated with TGF-β1, a potent stimulus of EMT. After 48 hr with TGF-β1, MDA-231^MYOFKD cells underwent an EMT. TGF-β

SUBMITTER: Barnhouse VR 

PROVIDER: S-EPMC5922389 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature