Development of a Click-Chemistry Reagent Compatible with Mass Cytometry.

ABSTRACT: The recent development of mass cytometry has allowed simultaneous detection of 40 or more unique parameters from individual single cells. While similar to flow cytometry, which is based on detection of fluorophores, one key distinguishing feature of mass cytometry is the detection of atomic masses of lanthanides by mass spectrometry in a mass cytometer. Its superior mass resolution results in lack of signal overlap, thereby allowing multiparametric detection of molecular features in each single cell greater than that of flow cytometry, which is limited to 20 parameters. Unfortunately, most detection in mass cytometry relies on lanthanide-tagged antibodies, which is ideal to detect proteins, but not other types of molecular features. To further expand the repertoire of molecular features th

SUBMITTER: Shaklee J 

PROVIDER: S-EPMC5923286 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature