Analysis of the Relationship between Alternative Respiration and Sterigmatocystin Formation in Aspergillus nidulans.

ABSTRACT: Aspergillus nidulans has one gene for alternative oxidase (EC 1.10.3.11). To investigate the relationship between this mitochondrial terminal oxidase and the formation of the mycotoxin sterigmatocystin, the encoding aodA gene was both deleted and overexpressed. Relative to the wild-type, the cyanide-resistant fraction of respiration in the late stationary stage—when sterigmatocystin production occurs—doubled in the overexpressing mutant carrying three aodA gene copies, but decreased to 10% in the deletant. Essentially identical results were obtained regardless whether the cultures were illuminated or protected from light. In contrast, sterigmatocystin yield in the aodA deletant was about half of that in the control when grown in the dark, while ao

SUBMITTER: Molnar AP 

PROVIDER: S-EPMC5923334 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature