Label-Free Biosensor Using a Silver Specific RNA-Cleaving DNAzyme Functionalized Single-Walled Carbon Nanotube for Silver Ion Determination.

ABSTRACT: Silver, a very common heavy metal, has been employed in electronics, medicine, jewelry, and catalysis due to its excellent chemical and physical characteristics. Silver-containing wastes can cause environmental pollution, so it is vital to monitor the Ag(I) concentration. Here, a label-free biosensor was developed for the Ag(I) detection, which used single-walled carbon nanotubes/field effect transistor (SWNTs/FET) to functionalize with a specific DNAzyme, containing an Agzyme and a complementary strand DNA (CS-DNA) embedded an RNA-base. The CS-DNA was covalently immobilized on the SWNTs’ surface through peptide bonds, and then combined with the Agzyme. When Ag(I) was bound with the Agzyme, the CS-DNA can be cleaved at the RNA site efficiently. The cleaved DNAzyme induced a remarkabl

SUBMITTER: Wang H 

PROVIDER: S-EPMC5923588 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature