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Effects of detraining and retraining on muscle energy-sensing network and meteorin-like levels in obese mice.

ABSTRACT: Increased intramuscular peroxisome proliferator-activated receptor gamma coactivator-1? (PGC-1?) with exercise directly or indirectly affects other tissues, but the effector pathway of PGC-1? has not been clearly elucidated. The purpose of this study was to investigate the effect of exercise and/or dietary change on the protein levels of the soleus muscle energy-sensing network and meteorin-like (Metrnl), and additionally to analyze the detraining and retraining effects in high-fat diet (HFD)-induced obese mice.One hundred male C57BL/6 mice were divided into normal-diet + sedentary (CO, n?=?20) and HFD?+?sedentary (HF, n?=?80) groups, and obesity was induced in the HF group through consumption of a 45% HFD for 6 weeks. The HF group was subdivided into HF only (n?=?20), HF?+?training (HFT, n?=?20), dietary change + sedentary (HFND, n?=?20), and HFND + training (HFNDT, n?=?20) groups, and the mice in the training groups underwent a treadmill training for 8 weeks, 5 times per week, 40 min per day. The HFT and HFNDT groups underwent 8-week training, 8-week detraining, and 4-week retraining.An 8-week training was effective in increasing the protein levels of soleus muscle AMP-activated protein kinase (AMPK), PGC-1?, and plasma Metrnl in the obese mice (P

SUBMITTER: Bae JY

PROVIDER: S-EPMC5924483 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Effects of detraining and retraining on muscle energy-sensing network and meteorin-like levels in obese mice.

Bae Ju Yong JY Woo Jinhee J Kang Sunghwun S Shin Ki Ok KO

Lipids in health and disease 20180427 1

<h4>Background</h4>Increased intramuscular peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) with exercise directly or indirectly affects other tissues, but the effector pathway of PGC-1α has not been clearly elucidated. The purpose of this study was to investigate the effect of exercise and/or dietary change on the protein levels of the soleus muscle energy-sensing network and meteorin-like (Metrnl), and additionally to analyze the detraining and retraining effects in hig ...[more]

PMID: 29703203

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Project description:BackgroundIn the context of mass regulation, 'muscle memory' can be defined as long-lasting cellular adaptations to hypertrophic exercise training that persist during detraining-induced atrophy and may facilitate future adaptation. The cellular basis of muscle memory is not clearly defined but may be related to myonuclear number and/or epigenetic changes within muscle fibres.MethodsUtilizing progressive weighted wheel running (PoWeR), a novel murine exercise training model, we explored myonuclear dynamics and skeletal muscle miRNA levels with training and detraining utilizing immunohistochemistry, single fibre myonuclear analysis, and quantitative analysis of miRNAs. We also used a genetically inducible mouse model of fluorescent myonuclear labelling to study myonuclear adaptations early during exercise.ResultsIn the soleus, oxidative type 2a fibres were larger after 2 months of PoWeR (P = 0.02), but muscle fibre size and myonuclear number did not return to untrained levels after 6 months of detraining. Soleus type 1 fibres were not larger after PoWeR but had significantly more myonuclei, as well as central nuclei (P < 0.0001), the latter from satellite cell-derived or resident myonuclei, appearing early during training and remaining with detraining. In the gastrocnemius muscle, oxidative type 2a fibres of the deep region were larger and contained more myonuclei after PoWeR (P < 0.003), both of which returned to untrained levels after detraining. In the gastrocnemius and plantaris, two muscles where myonuclear number was comparable with untrained levels after 6 months of detraining, myonuclei were significantly elongated with detraining (P < 0.0001). In the gastrocnemius, miR-1 was lower with training and remained lower after detraining (P < 0.002).ConclusionsThis study found that (i) myonuclei gained during hypertrophy are lost with detraining across muscles, even in oxidative fibres; (ii) complete reversal of muscle adaptations, including myonuclear number, to untrained levels occurs within 6 months in the plantaris and gastrocnemius; (iii) the murine soleus is resistant to detraining; (iv) myonuclear accretion occurs early with wheel running and can be uncoupled from muscle fibre hypertrophy; (v) resident (non-satellite cell-derived) myonuclei can adopt a central location; (vi) myonuclei change shape with training and detraining; and (vii) miR-1 levels may reflect a memory of previous adaptation that facilitates future growth.

Project description:PurposeThe aim of this study was to examine the effects of aerobic high-intensity training with reduced volume and training cessation on body composition and physical fitness after the end of season and the time required to recapture physical fitness with intensified retraining following two weeks of detraining in elite soccer players.MethodTwenty male semi-professional soccer players participated in this study. The soccer players were assigned to either a group that completed high-intensity aerobic training (HAT, n = 10) or to a detraining and retraining group (DHAT, n = 10) for a 5-week period immediately after the end of the season. The first 2 weeks of the period, members of the HAT group performed high-intensity aerobic exercise (80-90% of HRmax, 12 min × 3, three times per week), whereas members of the DHAT group abstained from any physical activity. During the subsequent 3 weeks, members of both the HAT and DHAT groups completed high-intensity aerobic exercise. Exercise performance testing and body composition analysis were performed before; after 2 weeks of detraining; and at 1, 2 and 3 weeks of retraining.ResultsIntensified high-intensity training for 5 weeks maintained the performance in the Yo-Yo Intermittent Recovery level 2 test (Yo-Yo IR2) and repeated sprints at any time point (P > 0.05). However 2 weeks of detraining resulted in significant decreases in the performance on the Yo-Yo IR2 (P < 0.01) and repeated sprints test (P < 0.05). Performance on the Yo-Yo IR2 enhanced after 2 weeks of retraining and was maintained up to 3 weeks after retraining, with no significant differences between conditions (P > 0.05). In addition, repeated sprint performance markedly decreased after the detraining period (P < 0.05) and was continuously lower compared to the baseline at 2 weeks after retraining (P < 0.05). Furthermore, this value reached baseline level at the end of the experimental period (P > 0.05). There were no significant differences between conditions in body composition, performance of agility, or sprint ability throughout the 5-week experimental period (P > 0.05).ConclusionsThe present data suggest that short-term detraining after the competitive season can markedly decrease performances in the Yo-Yo IR2 test and repeated sprints. To return to a previous level of ability on the Yo-Yo IR2 and/or sprint test with retraining through high-intensity aerobic training after a period of detraining, a similar or longer period of retraining is required. However, the high-intensity training with reduced amount of training after competitive season can prevent reductions in physical fitness.

Project description:Chemotherapy-induced peripheral neuropathy is a challenging condition to treat, and arises due to severe, dose-limiting toxicity of chemotherapeutic drugs such as paclitaxel. This often results in debilitating sensory and motor deficits that are not effectively prevented or alleviated by existing therapeutic interventions. Recent studies have demonstrated the therapeutic effects of Meteorin, a neurotrophic factor, in reversing neuropathic pain in rodent models of peripheral nerve injury induced by physical trauma. Here, we sought to investigate the potential antinociceptive effects of recombinant mouse Meteorin (rmMeteorin) using a paclitaxel-induced peripheral neuropathy model in male and female mice. Paclitaxel treatment (4 × 4 mg/kg, i.p.) induced hind paw mechanical hypersensitivity by day 8 after treatment. Thereafter, in a reversal dosing paradigm, five repeated injections of rmMeteorin (.5 and 1.8 mg/kg s.c. respectively) administered over 9 days produced a significant and long-lasting attenuation of mechanical hypersensitivity in both sexes. Additionally, administration of rmMeteorin ( .5 and 1.8 mg/kg), initiated before and during paclitaxel treatment (prevention dosing paradigm), reduced the establishment of hind paw mechanical hypersensitivity. Repeated systemic administration of rmMeteorin in both dosing paradigms decreased histochemical signs of satellite glial cell reactivity as measured by glutamine synthetase and connexin 43 protein expression in the dorsal root ganglion. Additionally, in the prevention administration paradigm rmMeteorin had a protective effect against paclitaxel-induced loss of intraepidermal nerve fibers. Our findings indicate that rmMeteorin has a robust and sustained antinociceptive effect in the paclitaxel-induced peripheral neuropathy model and the development of recombinant human Meteorin could be a novel and effective therapeutic for chemotherapy-induced peripheral neuropathy treatment. PERSPECTIVE: Chemotherapy neuropathy is a major clinical problem that decreases quality of life for cancer patients and survivors. Our experiments demonstrate that Meteorin treatment alleviates pain-related behaviors, and signs of neurotoxicity in a mouse model of paclitaxel neuropathy.

Dataset Information

Effects of detraining and retraining on muscle energy-sensing network and meteorin-like levels in obese mice.

Publications

Effects of detraining and retraining on muscle energy-sensing network and meteorin-like levels in obese mice.

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