Identification of a WNT5A-Responsive Degradation Domain in the Kinesin Superfamily Protein KIF26B.

ABSTRACT: Noncanonical WNT pathways function independently of the β-catenin transcriptional co-activator to regulate diverse morphogenetic and pathogenic processes. Recent studies showed that noncanonical WNTs, such as WNT5A, can signal the degradation of several downstream effectors, thereby modulating these effectors' cellular activities. The protein domain(s) that mediates the WNT5A-dependent degradation response, however, has not been identified. By coupling protein mutagenesis experiments with a flow cytometry-based degradation reporter assay, we have defined a protein domain in the kinesin superfamily protein KIF26B that is essential for WNT5A-dependent degradation. We found that a human disease-causing KIF26B mutation located at a conserved amino acid within this domain compromises the abilit

SUBMITTER: Karuna EP 

PROVIDER: S-EPMC5924538 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature