Two P_1B-1-ATPases of Amanita strobiliformis With Distinct Properties in Cu/Ag Transport.

ABSTRACT: As we have shown previously, the Cu and Ag concentrations in the sporocarps of Ag-hyperaccumulating Amanita strobiliformis are correlated, and both metals share the same uptake system and are sequestered by the same metallothioneins intracellularly. To further improve our knowledge of the Cu and Ag handling in A. strobiliformis cells, we searched its transcriptome for the P_1B-1-ATPases, recognizing Cu⁺ and Ag⁺ for transport. We identified transcripts encoding 1097-amino acid (AA) AsCRD1 and 978-AA AsCCC2, which were further subjected to functional studies in metal sensitive Saccharomyces cerevisiae. The expression of AsCRD1 conferred highly increased Cu and Ag tolerance to metal sensitive yeasts in which the functional AsCRD1:GFP

SUBMITTER: Benes V 

PROVIDER: S-EPMC5924815 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature