Individually addressable and dynamic DNA gates for multiplexed cell sorting.

ABSTRACT: The ability to analyze and isolate cells based on the expression of specific surface markers has increased our understanding of cell biology and produced numerous applications for biomedicine. However, established cell-sorting platforms rely on labels that are limited in number due to biophysical constraints, such as overlapping emission spectra of fluorophores in FACS. Here, we establish a framework built on a system of orthogonal and extensible DNA gates for multiplexed cell sorting. These DNA gates label target cell populations by antibodies to allow magnetic bead isolation en masse and then selectively unlock by strand displacement to sort cells. We show that DNA gated sorting (DGS) is triggered to completion within minutes on the surface of cells and achieves target cell purity, viabi

SUBMITTER: Dahotre SN 

PROVIDER: S-EPMC5924882 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature