Sepsis Induces a Dysregulated Neutrophil Phenotype That Is Associated with Increased Mortality.

ABSTRACT:

Background

Neutrophil dysfunction in sepsis has been implicated in the pathogenesis of multiorgan failure; however, the role of neutrophil extracellular traps (NETs) remains uncertain. We aimed to determine the sequential changes in ex vivo NETosis and its relationship with mortality in patients with sepsis and severe sepsis.

Methods

This was a prospective observational cohort study enrolling 21 healthy age-matched controls and 39 sepsis and 60 severe sepsis patients from acute admissions to two UK hospitals. Patients had sequential bloods for the ex vivo assessment of NETosis in response to phorbol-myristate acetate (PMA) using a fluorometric technique and chemotaxis using time-lapse video microscopy. Continuous data was tested for normality, with appropriate parametric and

SUBMITTER: Patel JM 

PROVIDER: S-EPMC5925119 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature