Identifying the minimum number of microsatellite loci needed to assess population genetic structure: A case study in fly culturing.

ABSTRACT: Small, isolated populations are constantly threatened by loss of genetic diversity due to drift. Such situations are found, for instance, in laboratory culturing. In guarding against diversity loss, monitoring of potential changes in population structure is paramount; this monitoring is most often achieved using microsatellite markers, which can be costly in terms of time and money when many loci are scored in large numbers of individuals. Here, we present a case study reducing the number of microsatellites to the minimum necessary to correctly detect the population structure of two Drosophila nigrosparsa populations. The number of loci was gradually reduced from 11 to 1, using the Allelic Richness (AR) and Private Allelic Richness (PAR) as criteria for locus removal. The effect of each re

SUBMITTER: Arthofer W 

PROVIDER: S-EPMC5927656 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature