A deep insight into the male and female sialotranscriptome of adult Culex tarsalis mosquitoes.

ABSTRACT: Previously, a Sanger-based sialotranscriptome analysis of adult female Culex tarsalis was published based on ∼2000 ESTs. During the elapsed 7.5 years, pyrosequencing has been discontinued and Illumina sequences have increased considerable in size and decreased in price. We here report an Illumina-based sialotranscriptome that allowed finding the missing apyrase from the salivary transcriptome of C. tarsalis, to determine several full-length members of the 34-62 kDa family, when a single EST has been found previously, in addition to identifying many salivary families with lower expression levels that were not detected previously. The use of multiple libraries including salivary glands and carcasses from male and female organisms allowed for an unprecedented insight into the tissue specifici

SUBMITTER: Ribeiro JMC 

PROVIDER: S-EPMC5927831 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature