Microbiota-Derived Metabolic Factors Reduce Campylobacteriosis in Mice.

ABSTRACT:

Background & aims

Campylobacter jejuni, a prevalent foodborne bacterial pathogen, exploits the host innate response to induce colitis. Little is known about the roles of microbiota in C jejuni-induced intestinal inflammation. We investigated interactions between microbiota and intestinal cells during C jejuni infection of mice.

Methods

Germ-free C57BL/6 Il10^-/- mice were colonized with conventional microbiota and infected with a single dose of C jejuni (10⁹ colony-forming units/mouse) via gavage. Conventional microbiota were cultured under aerobic, microaerobic, or anaerobic conditions and orally transplanted into germ-free Il10^-/- mice. Colon tissues were collected from mice and analyzed by histology, real-time polymerase chain reaction, and

SUBMITTER: Sun X 

PROVIDER: S-EPMC5927838 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature