A novel nano-immunoassay method for quantification of proteins from CD138-purified myeloma cells: biological and clinical utility.

ABSTRACT: Protein analysis in bone marrow samples from patients with multiple myeloma has been limited by the low concentration of proteins obtained after CD138⁺ cell selection. A novel approach based on capillary nano-immunoassay could make it possible to quantify dozens of proteins from each myeloma sample in an automated manner. Here we present a method for the accurate and robust quantification of the expression of multiple proteins extracted from CD138-purified multiple myeloma samples frozen in RLT Plus buffer, which is commonly used for nucleic acid preservation and isolation. Additionally, the biological and clinical value of this analysis for a panel of 12 proteins essential to the pathogenesis of multiple myeloma was evaluated in 63 patients with newly diagnosed multiple myeloma

SUBMITTER: Misiewicz-Krzeminska I 

PROVIDER: S-EPMC5927993 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature