A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors.

ABSTRACT: An insulator-based dielectrophoresis (iDEP) is a label-free method that has been extensively utilized for manipulation of nanoparticles, cells, and biomolecules. Here, we present a new iDEP approach that can rapidly trap nanoparticles at the close proximity of a glass nanopipette's tip by applying 10 V/cm direct current (DC) across the pipette's length. The trapping mechanism was systemically studied using both numerical modeling and experimental observations. The results showed that the particle trapping was determined to be controlled by three dominant electrokinetic forces including dielectrophoretic, electrophoretic and electroosmotic force. Furthermore, the effect of the ionic strength, the pipette's geometry, and the applied electric field on the entrapment efficiency was investigate

SUBMITTER: Shi L 

PROVIDER: S-EPMC5928082 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature