Direct binding of CEP85 to STIL ensures robust PLK4 activation and efficient centriole assembly.

ABSTRACT: Centrosomes are required for faithful chromosome segregation during mitosis. They are composed of a centriole pair that recruits and organizes the microtubule-nucleating pericentriolar material. Centriole duplication is tightly controlled in vivo and aberrations in this process are associated with several human diseases, including cancer and microcephaly. Although factors essential for centriole assembly, such as STIL and PLK4, have been identified, the underlying molecular mechanisms that drive this process are incompletely understood. Combining protein proximity mapping with high-resolution structural methods, we identify CEP85 as a centriole duplication factor that directly interacts with STIL through a highly conserved interaction interface involving a previously uncharacterised domain

SUBMITTER: Liu Y 

PROVIDER: S-EPMC5928214 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature