A functional genomic screen in vivo identifies CEACAM5 as a clinically relevant driver of breast cancer metastasis.

ABSTRACT: Tumor cells disseminate early in tumor development making metastasis-prevention strategies difficult. Identifying proteins that promote the outgrowth of disseminated tumor cells may provide opportunities for novel therapeutic strategies. Despite multiple studies demonstrating that the mesenchymal-to-epithelial transition (MET) is critical for metastatic colonization, key regulators that initiate this transition remain unknown. We serially passaged lung metastases from a primary triple negative breast cancer xenograft to the mammary fat pads of recipient mice to enrich for gene expression changes that drive metastasis. An unbiased transcriptomic signature of potential metastatic drivers was generated, and a high throughput gain-of-function screen was performed in vivo to validate candidates

SUBMITTER: Powell E 

PROVIDER: S-EPMC5928229 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature