Diversity of Growth Patterns Probed in Live Cyanobacterial Cells Using a Fluorescent Analog of a Peptidoglycan Precursor.

ABSTRACT: Cyanobacteria were the first oxygenic photosynthetic organisms during evolution and were ancestors of plastids. Cyanobacterial cells exhibit an extraordinary diversity in their size and shape, and bacterial cell morphology largely depends on the synthesis and the dynamics of the peptidoglycan (PG) layer. Here, we used a fluorescence analog of the PG synthesis precursor D-Ala, 7-Hydroxycoumarin-amino-D-alanine (HADA), to probe the PG synthesis pattern in live cells of cyanobacteria with different morphology. They displayed diverse synthesis patterns, with some strains showing an intensive HADA incorporation at the septal region, whereas others gave an HADA signal distributed around the cells. Growth zones covering several cells at the tips of the filament were present in some filamentous st

SUBMITTER: Zhang JY 

PROVIDER: S-EPMC5928242 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature